Riffyn: Measure Absorbance

Measure Absorbance Process

This process is showcased in our on-boarding videos and is an example of building a basic process. The process is here: https://app.riffyn.com/processes/gLY93cfcmvXPT6nH7

Riffyn’s Design Mode is where you will record process designs, which are distinct from experiments recorded in Measure Mode. An experiment is where you record your experimental data; a Process is the blueprint used when executing this experiment.

 

Design Mode

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Riffyn’s Design canvas is where you will build your process flow diagram by breaking your process into STEPS. Each step transforms input materials into output materials, and the movement of materials across steps creates linkages between these steps. These linkages will become the blueprint used for joining data in Measure mode.

The Measure Absorbance process has three steps:

  1. Get Dilution Buffer: Get dilution buffer(s) to be used for absorbance measurement. 
  2. Dilute Samples: Record sample(s) and standard(s) to be used in absorbance measurement.
  3. Measure Absorbance: Measure absorbance of solutions diluted in dilution buffer.

Input or output materials are generically called “resources” in Riffyn, and are shown in the bottom panel for each step:Screen_Shot_2017-06-07_at_3.33.09_PM.png

Resources can have properties and components associated with them.

Properties act as a placeholder for data, similar to a column in a spreadsheet. When an experiment is executed on a process, the data will be assigned to a particular property associated with a particular resource on a particular step. This hierarchy dictates how data are structured in measure mode.

Properties can be any type of data you intend to collect, ranging from procedural data, such as the serial number of your spectrophotometer, to  results data, such as the spectral absorbance of your output solution.

You can also assign target values to properties, such as the volumes of solution or buffer to be used in an experiment, along with upper and lower spec limits. Upon experiment execution in Measure Mode, a user can indicate whether target values were used, or whether these were overridden with different values.

Components are nested resources (see here for more explanation on that). For example, in this process, tris is a component of the buffer used, so the property “molar concentration” is associated with the tris component of the buffer, while the property “volume” is associated with the buffer resource itself:

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The side panel is where you will record your procedural tasks associated with each step of the process, and indicate workflow your operators:

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Design notes are where you can record important changes to the process and respond to colleague’s comments:

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Your work in Design mode is automatically saved, and often you will want to take snapshots of your process as versions so you can track changes over time. The version tab is where you can view all previous versions of the process and see on which versions your currently selected step was modified:

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Finally, a list of all the experiments executed on a process can be viewed from the Experiment drop down menu, and new experiments can be created from this menu as well:

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