The "Make Sample" feature can be used in two ways to represent your process in Design Mode.
When the same sample is measured in multiple steps
If you want samples to be tied together, then use a sampling step and the "make sample" feature. This means that the sample from the "Sample BR" step is measured on both the "Titer via HPLC" and "Offline Samples" steps:
When individual samples are used for each step
If you do not want individual sample sets (i.e. if the HPLC sample was pulled separate from the offline sample) to be associated then use sample of on the same output that the time course data is on: